Cross-allergenicity between milks from different ruminants are generally observed, thus rendering the use of goat’s and sheep’s milks (GSM) as alternative of cow’s milk (CM) unsafe for individuals sensitive to CM (3C5). strong class=”kwd-title” Keywords: milk, allergy, sheep, goat, IgE, -casein, ELISA, dairy product Introduction Milk is one of the Rabbit Polyclonal to MLH1 foods causing the most frequently an adverse reaction (1, 2). These quantitative assays could then be useful for the risk assessment of food products potentially contaminated with GM and SM in order to prevent adverse reactions in individuals specifically sensitive to these milks. Heat-treatment, ripening and coagulation processes, usually applied to dairy products that show a very high IgE-immunoreactivity, did not impair the test level of sensitivity. The checks were able to detect contaminations of GM in CM in the ppm level. Our two-site immunometric assays shown a high level of sensitivity with a detection limit of 1 1.6C3.2 ng/mL of caprine and ovine -caseins. Results were analyzed in relation with the IgE-immunoreactivity of the food matrices, thanks to sera from CM, GM/SM sensitive individuals. of two checks were identified with various dairy products. We as a result aimed to develop an assay able to detect traces of caprine/ovine -caseins in different food matrices, irrespective of the presence of the bovine homolog.